檢視染色体步移的原始碼

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|<center>'''染色体步移'''<br><img
src="https://gimg2.baidu.com/image_search/src=http%3A%2F%2Fbkimg.cdn.bcebos.com%2Fpic%2Fd31b0ef41bd5ad6ea9bc091b8bcb39dbb7fd3cfd&refer=http%3A%2F%2Fbkimg.cdn.bcebos.com&app=2002&size=f9999,10000&q=a80&n=0&g=0n&fmt=auto?sec=1653972906&t=a09fa482c806e38fde83e29a3c64e493 " width="280"></center><small>  圖片來自百度</small> 
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'''染色体步移''（Chromosome walking）是用于鉴定已经[[克隆]]的特定[[DNA]]片段侧翼顺序的方法。

从第一个重组克隆插入片段的一端分离出一个片段作为探针从文库中筛选第二个[[重组克隆]]，该克隆插入片段含有与探针重叠顺序和染色体的其他顺序。从第二个重组克隆的插入片段再分离出末端小片段筛选第三个重组克隆，如此重复，得到一个相邻的片段，等于在染色体上移了一步，故称之为染色体步移（Chromosome Walking）染色体步移技术（genome walking）是一种重要的[[分子生物学]]研究技术，使用这种技术可以有效获取与已知序列相邻的未知序列。

利用已知的基因或分子标记来筛选大片段的DNA文库获得[[阳性克隆]]，如此得到的阳性克隆是“一次克隆”，利用获得的克隆作为探针对文库进行第二次杂交，得到与[[探针]]具有重复序列的阳性克隆称为“二次克隆”，如此反复即可得到需要的克隆，获得目的基因。

*中文名:[[染色体步移]]
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|<center>'''染色体步移'''<br><img
src="https://gimg2.baidu.com/image_search/src=http%3A%2F%2Fm.wendangwang.com%2Fpic%2F22522ddbcb07a89cad73f1ff%2F9-810-jpg_6-1080-0-0-1080.jpg&refer=http%3A%2F%2Fm.wendangwang.com&app=2002&size=f9999,10000&q=a80&n=0&g=0n&fmt=auto?sec=1653973055&t=dd5d97e971f3e34a61a86f8532f13b9c " width="280"></center><small>  圖片來自百度</small> 
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*外文名:Chromosome walking

*别    名:[[基因组步移]]

*应用领域:[[医学鉴定]]