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[[File:应1385.jpg|缩略图|[http://faculty.dlut.edu.cn/1998011004/zh_CN/index.htm 原图链接]]] '''张西文''',男,现任中国遗传学会理事,中国化工学会生物工程分会理事,教育部科委生命科学学部委员,辽宁省遗传学会及生物技术学会理事。1998-2002年任大连理工大学生物工程系主任主要从事植物基因工程和药物生物技术研究,先后主持国家科技攻关项目1项、省、市级重大研究课题4项。 ==工作经历== 1998.3 至今[[大连理工大学[[ 教师 1984.10 1998.2[[辽宁师范大学]] 教师 1975.12 1978.2[[丹东市陶瓷厂]] 工人 1973.12 1975.12丹东市东沟县海洋红公社 知青 1972.12 1973.12丹东市凤城县赛马公社 知青 ==教育经历== 1986.9至1989.8 [[东北师范大学]]植物学博士 1982.3至1984.8 东北师范大学遗传学 硕士 1978.3至1982.2 [[辽宁师范大学]]生物学学士 1969.9至1972.8[[丹东市第二中学]]高中课程 <ref>[http://faculty.dlut.edu.cn/1998011004/zh_CN/index.htm 大连理工大学] </ref> ==社会兼职编== 2006.5至今评委(国家留学基金委) 2007.10至今副理事长(中国园艺学会小浆果分会) 2005.12020.12学部委员(教育部科委生命科学学部) 2005.12020.12理事(中国遗传学会) 2004.122019.12专家(辽宁省生物技术协会专家委员会) ==研究领域== 1. 植物转基因工程与育种技术平台研究。编号:JY03-B-18,国家科技部植物转基因重大专项,100万元,2003-2004 2. 基因工程关键技术。项目编号:2001101001,辽宁省科技厅,210万元,2001-2004 3. 无载体、无标记基因植物转基因技术。项目编号:2003B4NS015,大连市科技局,100万元,2003-2004 4. 蛇岛蝮蛇类凝血酶基因的分离和表达。辽宁省科技攻关,10万元,1999.01-2001.12 5. 蛇岛蝮蛇类凝血酶基因的分离和表达。大连市科委,15万元,1998-2001 6. 胸腺素α1基因工程药物开发。通化金马制药股份公司,390万元,1998.01~2001.12 7. 杨树基因工程育种。鞍山生生高新植物技术应用股份有限公司,25万元,2001.9-2003.9 8. 长春花碱单体的分离研究。大连理工大学领先集团有限公司,20万元,2002.7-2003.7 9. 真福康保健功效的验证与鉴定。大连理工大学领先集团有限公司,10万元,2002.7-2003.7 ==指导硕、博士生研究方向== 植物基因工程、生物制药 ==出版著作== 五年来共发表文章120余篇,SCI收录42篇,主要文章包括: 1. Properties of A. ficuum AS3.324 phytase expressed in tobacco. Process Biochemistry,2005,40:213–216 SCI 2. Purification,characterization and gene cloning of a novel phospholipase A2 from the venom of Agkistrodon blomhoffii ussurensis. The International Journal of Biochemical & Cell Biology. 2005,37:558-565 SCI 3. Neuroprotective properties of catalpol in transient global cerebral ischemia in gerbils: dose-response,therapeutic time-window and long-term efficacy. Brain Research,2004,1029:179-185 SCI 4. Plasmodium falciparum histone acetyltransferase,a yeast GCN5 homologue involved in chromatin remodeling. Eukaryotic Cell,2004,3(2):264-276 5. Catalpol inhibits apoptosis in hydrogen peroxide-induced PC12 cells by preventing cytochrome c release and inactivating of caspase casecade. Toxicon,2004,43:53-59 6. PfADA2,a Plasmodium falciparum homologue of the transcriptional coactivator ADA2 and its in vivo association with the histone acetyltransferase PfGCN5. Gene,2004,336:251-261 7. Construction of Targeting Vector and Expression of Green Fluorescent Protein in the Silkworm,Antheraea pernyi. DNA and Cell Biology. 2003,22:441-446 8. The Expression of GFP Under the Control of Fibroin Promotor in Primary ovarian cells of Antheraea pernyi. Journal of Biosciences,2003,28(6):691-695 9. 菟丝子提取物在PC12细胞株中的神经营养因子样活性。生物化学与生物物理进展。2003,30(2):226-230 10. Codonopsis pilosula (Franch) Nannf total alkaloids potentiate neurite outgrowth induced by nerve growth factor in PC12 cells. Acta Pharmacologica Sinica,2003,24(9):913-917 11. Effect of Cuscuta chinensis glycoside on the neuronal differentiation of rat pheochromocytoma PC12 cells. International Journal of Developmental Neuroscience,2003,21:277-281 12. Hydrogen peroxide-induced apoptosis in PC12 cells and the protective effect of puerarin. Cell Biology International,2003,27:1025-1031 13. Neuroprotective effect of Alpinia oxyphylla Miq. fruits against glutamate-induced apoptosis in cortical neurons. Toxicology Letters,2003,144(2):205–212 14. Neuroprotective effect of Alpinia oxyphylla extract against glutamate excitotoxicity in cultured mouse cortical neurons. Neurosci Res Commun,2003,33(2):105–113 15. Investigation of the neuronal death mode induced by glutamate treatment in serum-,antioxidant-free primary cultured cortical neurons. Developmental Brain Research,2003,145(2):263-268 16. A new way for chemical degradation of plastic by natural volatile constituents of Ledum palustre. Chinese Science Bulletin,2003,48(16):1718-1721 17. Enhancing ethanol tolerance of a self-flocculating fusant of Schizosaccharomyces pombe and Saccharomyces cerevisiae by Mg2+ via reduction in plasma membrane permeability. Biotechnology Letters,2003,25:1191-1194 18. Dextromethorphan protects dopaminergic neurons against inflammation-mediated degeneration through inhibition of microglial activation. The Journal Of Pharmacology and Experimental Therapeutics,2003,305(1):212-218 19. 酸性水溶液中硫氰酸钼(V)配合物的研究。光谱学与光谱分析,2003,23(2)337-339 20. Purification and characterization of ulva pertusa kjellm alkaline phosphatase. Preparative Biochemistry & Biotechnology,2003,33(2):113-123 21. Cloning of cDNA Encoding Choline Monooxygenase from Suaeda liaotungensis and Salt Tolerance of Transgenic Tobacco. Acta Botanica Sinica,2003,45(2):242-247 22. Molecular cloning and characterization of betaine aldehyde dehydrogenase gene from Suaeda liaotungensis and its use in improved tolerance to salinity in transgenic tobacco. Biotechnol Lett. 2003,25(17):1431-1436 23. Characterization of Sporothric schenckii by random amplication of polymorphic DNA assay. Chinese Medicine Journal,2003,116(2):239-242 24. Expression of gloshedobin,a thrombin-like enzyme from the venom of Gloydius shedaoensis,in Escherichia coli. Biotechnology Letters,2003,25(2):101–104 25. High-level expression of a soluble snake venom enzyme,gloshedobin,in E. coli in the presence of metal ions. Biotechnology Letters,2003,25(8):607–610 26. Purification and characterization of ginsenoside--arabinofuranase hydrolyzing ginsenoside Rc into Rd from the fresh root of Panax ginseng. Process Biochemistry,2002,37:793-798 27. Cloning and Expression of defibrase cDNA from the venom of Gloydius shedaoensis. Biotechnology Letters,2002,24:135- 138 28. Cloning Expression and Purification of Gussrobin A Thrombin-like Enzyme from the Snake Venom of Gloydius ussuriensis. Prog Biochem Biophys. 2002,34(1):6-10 29. Expression,Purification and Partial Characterization of Recombinant Defibrase,a Thrombin-Like Enzyme from the Venom of Glyodius shedaoensis. Prog Biochem Biophys. 2002,29(3)390-393 30. Production of a Functional Single-chain Fv Protein in Transgenic Tobacco Root Exudates. Biotechnology letters. 2002,24(18):1531-1534 31. An expeditious method for constructing T-vectors using Eam 1105 Ⅰ cassettes. Plant Molecular Biology Reporter,2002,20:189a-189e 32. Application of DNA sequencing in detection of telomerase activity. Biochemical Engineering Journal. 2002,11(2- 3):79-85 33. Production of high affinity human single-chain antibody against preS1 of Hepatitis B Virus: comparison of large naïve and in vitro immune phage displayed antibody library. Progressing in Biochemistry and Biophysics. 2002,29 (4):572-575 34. Inhibition by naloxone stereoisomers of beta-amyloid peptide (1-42)-induced superoxide production in microglia and degeneration of cortical and mesencephalic neurons. Journal of Pharmacology and Experimental Therapeutics,2002,302 (3):1212-1219 35. A Serum- and Antioxidant-free Primary Culture Model of Mouse Cortical Neurons for Pharmacological Screen and Studies of Neurotrophic and Neuroprotective Agents. Cellular and Molecular Neurobiology. 2002,22(2):197-206 36. Expression of endoplasmic reticulum molecular chaperon GRP94 in human lung cancer tissues and its clinical significance. Chinese Medical Journal,2002,115(11):1615-1619 37. Embryonic stem cell as nuclear donor could promote in vitro development of the heterogeneous reconstructed embryo. Chinese Science Bulletin,2002,47(21):1811-1815 38. 铜锌超氧化物歧化酶与色氨酸钴(Ⅱ)的相互作用。光谱学与光谱分析,2000,3:305-307 39. 紫外-可见吸收光谱法测定配合显色反应的焓变。光谱学与光谱分析,2001,21(4):527-528 40. Kinetics of Inactivation of Ulva pertusa Kjellm Alkaline Phosphatase by Ethylenediaminetetraacetic Acid Disodium. Journal of Enzyme Inhibition,2001,16:313-319 41. Purification and Characterization of ginsenoside-?-Glucosidase from Ginseng. Chem. Pharm. Bull,2001,49(7):795- 798 42. 分光光度法研究EDTA对小牛肠碱性磷酸酶的不可逆抑制动力。光谱学与光谱分析,2001,21(5):701-703 43. 碱性磷酸梅与Cu (Ⅱ) 相互作用的谱学研究。光谱学与光谱分析,2001,21(4):432-434 44. 氨基酰化酶与Cu(Ⅱ)相互作用的谱学研究。光谱学与光谱分析,2001,21(6):804-806 45. 可见光谱学研究核酸酶P1与氯化铜(Ⅱ)的相互作用。光谱学与光谱分析,2000,20(3):308-310 46. 大连蛇岛蝮蛇类凝血酶基因克隆与表达研究。大连理工大学学报,2003,43(2):156-159 47. 新的重组蛋白表达系统——植物根分泌和叶分泌。生物化学与生物物理进展,2003,30(1):128 48. 抗乙肝病毒表面抗原PreS1(20-47)的单链抗体在转基因烟草根分泌物中的初步表达。高技术通讯,2003,13(9):35-38 49. Variation and characterization analysis of partial fragment of fibroin gene from silkworm,Antheraea pernyi. High Technology Letters,2003,9(3):29-32 50. 喜树碱生物合成途径及其代谢调控的研究进展。高技术通讯,2004,14(5):106-110 51. 菟丝子提取物对PC12细胞中GAP-43表达影响。大连理工大学学报,2004,44(3):378-382 52. Rapid Amplification of 5′cDNA End of Suaeda liaotungensis Choline Monooxygenase Using Inverse PCR RACE. High Technol Lett 2002,8(1):5-7 53. Separation and purification of betaine aldehyde dehydrogenase from wild Suaeda liaotungensis. High Technology Letters,2002,8(2):5-17 54. Cloning a Full-length cDNA Encoding UDP-glucose Pyrophosphorylase from Amorpha Fruticosa by PCR-Based Methods. High Technology Letters,2002,8(3):5-10 55. An Lijia. A co-expression system based on phage and phagemid to select cognate antibody-antigen pairs in vivo. High Technology Letters,2002,8(2):5-10 56. Construction of Large Human Single-chain Antibody Phage Display Library. High Technology Letters,2002,8(3):1-4 57. 精子干细胞转染法制备转基因兔的研究。高技术通讯,2001,11(130):17-21 58. 反向嵌套PCR高效扩增辽宁碱蓬甜菜碱醛脱氢酶cDNA 5’末端序列。高技术通讯,2001,1(11):17-19 59. Research on telomerase detection of activity by combining DNA sequence analysis with TRAP. High Technology Letters,2001,7(3):8-10 60. 新型染料配基对碱性磷酸酶的亲合纯化。高校化学工程学报,2001,5(6):563-567 61. Separation and Purification of Thrombin-like Enzyme by Affinity Adsorbents. High Technology Letters,2001,7(4): 13-15 62. 卵黄免疫球蛋白配基亲和纯化碱性磷酸酶。中国农业科学,2004,37(6):908-911 ==参考资料== {{Reflist}} [[Category:教师]]
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