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刘奋勇

刘奋勇，芝加哥大学博士，耶鲁大学博士后，2006年从美国加州应聘来到中国医药城，担任市病毒研究所所长。

目录

简介

去年他从美国加州应聘来到中国医药城，担任市病毒研究所所长。作为引进的高层次人才，他去年底获得了100万元的省级高层次创业创新人才计划资助。

刘奋勇
 
刘奋勇
国籍 美国
职业 病毒学专家

人物生平

刘奋勇，病毒学、生物化学专业，美国籍，现为美国加州大学柏克利分校公共卫生学院终生教授，同时还任加大柏克利分校比较生物化学学科主任。刘奋勇博士先后师从美国科学院院士、世界著名的疱疹病毒专家、芝加哥大学 Bernard Roizman教授和诺贝尔奖获得者、耶鲁大学Sidney Altman教授。 刘奋勇博士目前主要从事HCMV研究以及治疗这类病的新药物和方法的研究与开发;在人疱疹病毒的基因组和功能组研究方面和由核酶介导的基因治疗方面取得了重要的研究成果;先后在美国科学院院刊、Journal of Virology、Cell、Gene & Development、Nucleic Acids Research等国际一流和知名学术刊物上发表研究论文数十篇;并获得"美国杰出青年学者奖"、"美国白血病协会杰出学者奖"、"美国心脏协会杰出学者奖"等。

研究领域

刘奋勇博士主要从事病毒学与抗病毒药物领域的研究，在国内外著名学术刊物共发表六十多篇学术论文和理论著作。研究成果在美国注册专利五项。现任美国国家健康研究院(NIH)基金委员会正式评委和美国癌症协会基金委员会正式评委。

2006年12月从美国加州引入江苏。到泰州市病毒研究所从事技术开发与服务工作，主要从事快速诊断禽流感病毒株的芯片产品的开发, 设计, 生产及销售。在最初两年试销和市场培育期内，每年将生产及售出至少20,000套人类芯片和20,000套动物芯片。预计今后几年内，每年将售出200,000套人类芯片和200,000套动物芯片。此外，帮助引进多名国内外知名专家担任研究所顾问。

擅长领域

医院药房不仅是医院医疗服务的重要窗口，也是医院重要的经济部门，而目前随着国家医疗制度的改革和医疗保险制度的出台，各级医院面临激烈的竞争。

医院药房应做到以下几点制定完善的规章制度、改善药房的工作环境、加强药房的硬件设施、以患者为中心、医院药学管理微机化、注重人才培养提高药学人员素质、接受社会监督，方能适应新形势，为医院整体的生存和发展做贡献。

研究成果

在甲型H1N1流感肆虐的时刻，中国医药城的入园企业-泰州亲和力生物技术有限公司自主研发的流感病毒检测芯片，已顺利通过省药监局医疗器械生产条件许可证现场验收，该芯片可 在6小时内对甲型H1N1流感疑似病例进行确诊。 该病毒检测芯片由美国芝加哥大学博士、耶鲁大学博士后，美国加州大学柏克利分校公共卫生学院终身教授、比较生物化学学科主任刘奋勇研发。2008年1月，刘奋勇博士加盟中国医药城，注册了高新技术企业--泰州亲和力生物技术有限公司，建立了先进的分子生物学实验室、生物二级动物房和GMP芯片生产厂房，主要从事严重危害人类健康的病毒性疾病的快速诊断、病原分子生物学及药物防治研究。经过近一年的努力，企业研制出了 "世界第一"也是"世界唯一"的流感病毒诊断生物芯片。

该芯片采用国际上最新的基因芯片技术，攻克了探针设计、信号放大、数据分析等一系列技术难关，具备快速便捷、高通量、一块芯片分型检测多种病毒等特点，能把流感诊断时间从过去的几天缩短为几个小时，像人源、禽源等多种属来源流感病毒2个小时内即可检测，时下肆虐的甲型H1N1流感6小时内就可检测确诊。

在检测甲型H1N1流感病毒时，只需对可疑患者鼻或咽拭子标本 (用医用棉签从人体鼻或咽部蘸取少量分泌物)、漱口水进行检测。芯片具有灵敏度高、特异性强、检测速度快、样本前处理相对简单、价格低、携带方便等优点，填补了国际同行业空白。

折叠编辑本段人物贡献 美国芝加哥大学博士、耶鲁大学博士后;美国加州大学柏克利分校公共卫生学院终身教授、比较生物化学学科主任;病毒学、生物化学方面的领军人才，研究成果在美国注册五项专利。

拥有这么多令人咋舌头衔的人，叫刘奋勇，泰州市病毒研究所所长，"江苏省高层次创业创新人才引进计划"首批引进人才。 在泰州，他仅用一年多时间，就研究出世界上第一块禽流感诊断生物芯片，把禽流感诊断时间从过去的7天缩短为2小时以内，为抢救病人赢得了时间。

当有专家指出这项新技术是世界第一时，他笑了:"这不仅是世界第一，而且是世界唯一。"这个世界唯一，采用了国际上最新的基因芯片技术，攻克了探针设计、信号放大、数据分析等一系列技术难关，具备快速便捷、高通量、一块芯片上分型检测多种病毒等三大特点，对指导临床诊疗和预防监测流感、保障公共安全提供了强有力的技术支撑。

在泰州医药园，刘奋勇针对医药产品研发周期长的特点，在较短时间内建立了完善的科技创新平台，研究所注册了中美合资的高新技术企业--泰州亲和力生物技术有限公司，建立了先进的分子生物学实验室、生物安全二级动物房和GMP芯片生产厂房，还先后招聘了包括博士、硕士和教授在内15名高层次人才，专业涵盖生物化学、病毒学、免疫学和分子生物学等领域，紧盯学科的国际发展前沿，取得一系列技术突破和阶段性成果。 "中国医药城是我事业的一个新起点，这里不仅有创业最佳的平台，优厚的人才政策，周到细致的服务，更有家一般的温暖。"刘奋勇说，他将努力创造更多的"世界第一、世界唯一"来回报江苏，为祖国的发展尽心尽力

 
刘奋勇1

主要论文

1. Liu, F., and Roizman, B. (1991). The promoter, transcriptional unit, and coding sequence of herpes simplex virus 1 family 35 proteins are contained within and in frame with the UL26 open reading frame. J. Virol. 65: 206-212.

2. Liu, F., and Roizman, B. (1991). The herpes simplex virus 1 gene encoding a protease also contains within its coding domain the gene encoding the more abundant substrate. J. Virol. 65: 5149-5156.

3. Liu, F., and Roizman, B. (1992). Differentiation of multiple domains in the herpes simplex virus 1 protease encoded by the UL26 gene. Proc. Natl. Acad. Sci. USA. 89: 2076-2080.

4. DiIanni, C. L., Drier, D. A., Deckman, I.C., McCann III, P. J., Liu, F., Roizman, B., Colonno, R. J. and Cordingley, M. G. (1993). Identification of the herpes simplex virus 1 protease cleavage sites by direct sequence analysis of autoproteolytic cleavage products. J. Biol. Chem. 268: 2048-2051.

5. Liu, F., and Roizman, B. (1993). Characterization of the protease and of other products of the amino terminus proximal cleavage of the herpes simplex virus 1 UL26 protein. J. Virol. 67, 1300-1309.

6. Liu, F., and Altman, S. (1994). Differential evolution of substrates for an RNA enzyme in the presence and absence of its protein cofactor. Cell, 77, 1093-1100.

7. Liu, F., and Altman, S. (1995). Inhibition of viral gene expression by the catalytic RNA subunit of RNase P for Escherichia coli. Genes & Development, 9, 471-480.

8. Liu, F., and Altman, S. (1996). Requirements for cleavage by a modified RNase P of a small model substrate. Nucleic Acids Res. 24, 2690-2696.

9. Kim, J., Kilani, A., Zhan, X., Altman, S., and Liu, F. (1997). The protein cofactor allows the sequence of an RNase P ribozyme to diversify by maintaining the catalytically active structure of the enzyme. RNA, 3, 613-623.

10. Yuan, Y., and Liu, F. (1998). Targeted cleavage of RNA using eukaryotic RNase P and external guide sequences. In Therapeutic applications of ribozymes. Edited by K. J. Scanlon, Methods in Molecular Medicine . Humana Press, New Jersey. p397-414.

11. Kawa, D., Wang, J., Yuan, Y., and Liu, F. (1998). Inhibition of viral gene expression by human ribonuclease P. RNA. 4: 1397-1406.

12. Chen, D., Jiang, H., Lee, M., Liu, F., and Zhou, H. (1999). Three-dimensional visualization of tegument/capsid interactions in intact human cytomegalovirus. Virology, 260, 10-16.

13. Kilani, A. F. and Liu, F. (1999). UV-crosslink mapping of the substrate-binding site of RNase P ribozyme to a target mRNA sequence. RNA, 5, 1235-1247.

14. Trang, P., Hsu, A., and Liu, F. (1999). Nuclease footprint analyses of the interactions between RNase P ribozyme and a model mRNA substrate. Nucleic Acids Res. 27, 4590-4597.

15. Zhan, X., Abenes, G., Lee, M., VonReis, I. , Kittikarookoon, C., Ross-Macdonald, P., Snyder, M., and Liu, F. (2000). Mutagenesis of murine cytomegalovirus using a Tn3-based transposon. Virology, 266, 264-274.

16. Kilani, A. F., Trang, P., Jo, S. Hsu, A., Kim, J., Nepomuceno, E., and Liu, F. (2000). RNase P ribozymes selected in vitro to cleave a viral mRNA effectively inhibit its expression in cell culture. J. Biol. Chem.,275, 10611-10622.

17. Wang, J., Jiang, H., and Liu, F. (2000). In vitro selection of novel RNA ligands that bind human cytomegalovirus and block viral infection. RNA, 6, 571-583.

18. Trang, P., Lee, M., Nepomuceno, E., Kim, J., Zhu, H., and Liu, F. (2000). Effective inhibition of human cytomegalovirus gene expression and replication by a RNase P ribozyme. Proc. Natl. Acad. Sci. USA, 97, 5812-5817.

19. Liu, F. , Wang, J., and Trang, P. (2000). In vitro selection of substrates by RNase P ribozymes. Methods in Enzymology, Vol 318, 238-250.

20. Zhan, X., Lee, M., Xiao, J., and Liu, F. (2000). Construction and characterization of murine cytomegalovirus mutants that contain a transposon insertion at open reading frames m09 and M83. J. Virol. 74, 7411-7421.

21. Hsu, A. W., Kilani, A. F., Liou, K., Lee, J., and Liu, F. (2000). Differential effects of the protein cofactor on the interactions between an RNase P ribozyme and its target mRNA substrate. Nucleic Acids Res. 28, 3105-3116.

22. Trang, P., Kilani, A. F., Lee, J., Kim, J., and Liu, F. (2000). A ribozyme derived from the catalytic subunit of RNase P from Escherichia coli is highly effective in inhibiting replication of herpes simplex virus 1. J. Mol. Biol.,301, 817-826.

23. Xiao, J., Tong, T., Zhan, X., Haghjoo, E., and Liu, F. (2000) In vitro and in vivo characterization of a murine cytomegalovirus with a transposon insertional mutation at open reading frame M43. J. Virol., 74, 9488-9497.

24. Lee, M., Xiao, J., Haghjoo, E., Tong, T., Abenes, G., Zhan, X., Dunn, W., and Liu, F. (2000) Murine cytomegalovirus containing a mutation at open reading frame M37 is severely attenuated in growth and virulence in vivo. J. Virol. 74, 11099-11107.

25. Abenes, G., Haghjoo, E., Lee, M., Tong, T., Zhan, X., and Liu, F. (2001) Murine cytomegalovirus open reading frame M27 plays an important role in viral growth and virulence in mice. J. Virol. 65, 1697-1707.

26. Trang,P., Lee, J., Kilani, A. F., Kim, J., and Liu, F. (2001) Effective inhibition of herpes simplex virus 1 gene expression and growth by engineered RNase P ribozyme. Nucleic Acids Res, 29, 5071-5078.

27. Dunn, W., Trang. P., Kahn, U., Nassi, A., and Liu, F. (2001). RNase P-mediated inhibition of cytomegalovirus protease expression and viral DNA encapsidation by oligonucleotide external guide sequences. Proc. Natl. Acad. Sci. USA, 98, 14831-14836.

28. Trang, P., Hsu, A., Zhou, T., Lee, J., Kilani, A. F., Nepomuceno, E., and Liu, F. (2002). Engineered RNase P ribozymes inhibit gene expression and growth of cytomegalovirus by increasing rate of cleavage and substrate binding. J. Mol. Biol. 315, 573-586.

29. Zhou, T., Kim, J., Kim, K., Kilani,A. F., Dunn, W., Jo, S., Nepomuceno, E., and Liu, F. (2002). In vitro selection of external guide sequences for RNase P-mediated inhibition of viral gene expression. J. Biol. Chem. 277, 30112-30120.

30. Trang, P., Kilani, A., Lee, J., Hsu, A., Liou, K., Khan, U., Nassi, A., and Liu, F. (2002). (Review) RNase P ribozymes for the studies and treatment of human herpesvirus infections. Journal of Clinical Virology, 25, 63-74.

31. Lee, M., Abenes, G., Zhan, X., Xiao, J., Dunn, W., Haghjoo, E., Tong, T., Kim, J., Tam, A., and Liu, F. (2002) (Review). Genetic analyses of gene function and pathogenesis of murine cytomegalovirus by transposon-mediated mutagenesis. Journal of Clinical Virology, 25, 111-122.

32. Zhu, J., Chen, J., Hai, R., Tong, T., Xiao, J., Zhan, X., Lu, S., and Liu, F. (2003). In vitro and in vivo characterization of a murine cytomegalovirus with mutation at open reading frame m166. J. Virol. 77, 2882-2891.

33. Trang, P., Kim, K., Zhu, J., and Liu, F. (2003). Expression of a RNase P ribozyme against the mRNA encoding human cytomegalovirus protease inhibits viral capsid protein processing and growth. J. Mol. Biol. 328, 1123-1135..

34. Tam, A., Zhu, J., Hai, R., Haghjoo, E., Zhou, T., Zhan, X., and Liu, F. (2003). Murine cytomegalovirus with a transposon insertional mutation at open reading frame M35 is defective in growth in vivo. J. Virol. 77, 7745-7755.

35. Singh, R., Haghjoo, E., and Liu, F. (2003). Cytomegalovirus M43 gene modulates T helper cell response. Immunol Lett. 88, 31-5.

36. Zou, H., Lee, J., Umamoto, S., Kilani, A. F., Kim, J., Trang, P., and Liu, F. (2003). Engineered RNase P ribozymes are efficient in cleaving a human cytomegalovirus mRNA in vitro and are effective in inhibiting viral gene expression and growth in human cells. J. Biol. Chem., 278, 37265-37274.

37. Raj, M. L. S., and Liu, F. (2003) (review). Engineering of RNase P for gene-targeting applications. Gene. 313, 59-69.

38. Dunn, W., Chou, C., Li, H., Hai, R., Patterson, D., Stolc, V., Zhu, H., and Liu, F. (2003). Functional profiling of human cytomegalovirus genome. Proc. Natl. Acad. Sci. USA. 100, 14223-14228.

39. Zou, H., Chan, K., Trang, P., and Liu, F. (2004). (Book chapter). General design and construction of RNase P ribozymes for gene-targeting applications. In Catalytic Nucleic Acids. Edited by M. Sioud and J. Walker, Methods in Molecular Medicine. Vol. 252, p385-398. Humana Press, New Jersey.

40. Kim, K., and Liu, F. (2004). (Book chapter). In vitro selection of RNase P ribozymes that efficiently cleave a target mRNA. In Catalytic Nucleic Acids. Edited by M. Sioud and J. Walker, Methods in Molecular Medicine Vol. 252, p399-412. Humana Press, New Jersey.

41. Raj, M. L., and Liu, F. (2004). (Book chapter). In vitro selection of external guide sequences for directing human RNase P to cleave a target mRNA. In Catalytic Nucleic Acids. Edited by M. Sioud and J. Walker, Methods in Molecular Medicine . Vol 252, p413-424. Humana Press, New Jersey.

42. Dunn, W., and Liu, F. (2004). (Book chapter). RNase P-mediated inhibition of viral growth by exogenous administration of short oligonucleotide external guide sequence. In Catalytic Nucleic Acids. Edited by M. Sioud and J. Walker, Methods in Molecular Medicine. Vol 252, p425-436. Humana Press, New Jersey.

43. Trang, P. and Liu, F. (2004). (Book chapter). RNAse P ribozyme as an antiviral agent against human cytomegalovirus. In Catalytic Nucleic Acids. Edited by M. Sioud and J. Walker, Methods in Molecular Medicine . Vol. 252, p437-450. Humana Press, New Jersey.

44. Kim, K., Umamoto, S., Trang, P., Hai, R., and Liu, F. (2004). Intracellular expression of engineered RNase P ribozymes effectively blocks gene expression and replication of human cytomegalovirus. RNA, 10, 438-447.

45. McGregor, A., Liu, F., and Schleiss, M. R. (2004). Identification of essential and non-essential genes of the guinea pig cytomegalovirus (GPCMV) genome via transposome mutagenesis of an infectious BAC clone. Virus Res. 101, 101-8.

46. Trang, P., Kim, K., and Liu, F. (2004) (Invited review). Developing RNase P ribozymes for gene-targeting and antiviral therapy. Cellular Microbiology, 6, 499-508.

  • 47. Abenes, G., Chan, K., Haghjoo, K., Zhu, J., Lee, M., Zhou, T., Tong, T., and Liu, F. (2004). A murine cytomegalovirus with a transposon insertional mutation at open reading frame m155 is attenuated in growth and virulence in immunodeficient hosts. J. Virol. 78, 6891-6899.
  • 48. Zhu, J., Trang P., Kim, K., Zhou, T., Deng, H., and Liu, F. (2004). Effective inhibition of Rta expression and lytic replication of Kaposi's sarcoma-associated herpesvirus by human RNase P. Proc. Natl. Acad. Sci. USA. 101, 9073-9078.
  • 49. Kim, K., Trang, P., Umamoto, S. Hai, R., and Liu, F. (2004). RNase P ribozyme inhibits cytomegalovirus replication by blocking the expression of viral capsid proteins. Nucleic Acids Res., 32, 3427-34.
  • 50. Lee, M., and Liu, F. (2004). (Book chapter). Genetic analysis of cytomegalovirus using shuttle mutagenesis. In DNA Viruses: Methods and Protocols. Edited by Lieberman, P., Methods in Molecular Biology. Humana Press, New Jersey. 292, 371-386.
  • 51. Zou, H., Lee, J., Kilani, A. K., Kim, K., Trang, P., Kim, J., Liu, F. (2004). Engineered RNase P ribozymes increase their cleavage activities and efficacies in inhibiting viral gene expression in cells by enhancing the rate of cleavage and binding of the target mRNA. J. Bio. Chem. 279, 32063 - 32070.
  • 52, McGregor, A., Liu, F., and Schleiss, M. R. (2004). Molecular, biological, and in vivo characterization of the guinea pig cytomegalovirus homologs of the HCMV matrix proteins pp71 (UL82) and pp65 (UL83). J. Virol. 78, 9872-9889.
  • 53. Lodoen, M., Abenes, G., Umamoto, U., Houchins, J. P., Liu, F., and Lanier, L. L. (2004). The Cytomegalovirus m155 Gene Product Subverts NK cell Antiviral Protection by Disruption of H60-NKG2D Interactions. J. Exp. Med. 200, 1075-1081.
  • 54. Yu, X., Shah, S., Atanasov, I., Lo, P., Liu, F., Britt, W., and Zhou, Z. H. (2005). Three-dimensional localization of smallest capsid protein in human cytomegalovirus capsid. J. Virol. 79, 1327-1332.
  • 55. Netterwald, J., Yang, S.,Wang, W., Ghanny, S., Cody, M., Soteropoulos, P., Bin,T., Dunn, W., Liu, F., and Zhu, H. (2005). Two VRS elements in the human cytomegalovirus major immediate-early promoter/enhancer are important for viral replication. J. Virol. 79, 5035-46.
  • 56. Yu, X., Trang, P., Shah, S., Atanasov, I., Bai, Y., Zhou, Z. H. and Liu, F. (2005). Dissecting human cytomegalovirus gene function and capsid maturation by ribozyme targeting and electron cryomicroscopy. Proc. Natl. Acad. Sci. USA, 102, 7103-7108.
  • 57. Dunn, W., Trang, P., Zhong, Q., Yang, W., van Belle, C., Bai, Y., and Liu, F. (2005). Human cytomegalovirus expresses novel microRNAs during productive viral infections. Cell Micro., 7, 1684-1695.
  • 58. Li, H., Trang, P., Kim, K., Zhou, T., and Liu, F. (2006). Effective inhibition of human cytomegalovirus gene expression and growth by intracellular expression of external guide sequence RNA. RNA, 12, 63-72.
  • 59. Yang, Y. H., Li, H., Zhou, T., Kim, K., and Liu, F. (2006). Engineered external guide sequences are highly effective in inducing RNase P for inhibition of gene expression and replication of human cytomegalovirus. Nucleic Acids Res. 34, 575-583.
  • 60. Yang, S., Ghanny, S., Wang, W., Galante, A., Dunn, W., Liu, F., Soteropoulos, P., and Zhu, H. (2006). Using DNA microarray to study human cytomegalovirus gene expression, J. Virol. Methods. 131, 202-208.
  • 61. Hai, R., Chu, A., Li, H., Umamoto, S., Rider, P., Liu, F. (2006). Infection of human cytomegalovirus in cultured human gingival tissue. Virology J. 3:84.
  • 62. Liu, F., and Zhou, Z. H. (2007). (Book chapter). Comparative virion structures of human herpesviruses. In Human herpesviruses: pathogenesis, therapeutics, and immunophalactics. Edited by Arvin, A., Mocarski, E., Moore, P., Roizman, B., Whitley, R., Yamaguchi, P. Cambridge University Press, New York. p27-43.
  • 63. Kim, K. and Liu, F. (2007). (Invited review) Inhibition of gene expression in human cells using RNase P-derived ribozymes and external guide sequences. Biochimica et Biophysica Acta. 1769, 603-612.
  • 64. Bai, Y., Trang, P., Li, H., Kim, K., Zhou, T., and Liu, F. (2008). Effective inhibition in animals of viral gene expression and pathogenesis by an engineered ribozyme derived from RNase P catalytic RNA. Proc. Natl. Acad. Sci. USA. 105, 10919-24.
  • 65. Trang, P. and Liu, F. (2008). (Book chapter). Mapping the regions of RNase P RNA that are potentially in close contact with its protein cofactor. Methods Mol Biol., 488, 267-277.
  • 66. Rider, P. J., Dunn, W., Yang, E., and Liu, F. (2008). Human cytomegalovirus microRNAs. Curr Top Microbiol Immunol, 325, 21-39.
  • 67. Qi, X., Li, X., Rider, P., Fan, W., Gu, H., Xu, L., Yang, Y., Lu, S., Wang, H., and Liu, F. (2009) Molecular Characterization of Highly Pathogenic H5N1 Avian Influenza A Viruses Isolated from Raccoon Dogs in China. PLoS ONE, 4, e4682.
  • 68. Gong, H., Su, J., Bai, Y., Miao, L., Kim, K., Yang, Y., Liu, F., and Lu, S. (2009). Characterization of the expression of Salmonella Type III secretion system factor PrgI, SipA, SipB, SopE2, SpaO, and SptP in cultures and in mice. BMC Microbiology. 9, 73.
  • 69. Li, X., Qi, X., Miao, L., Wang, Y., Liu, F., Gu, H., Lu, S., Yang, Y., Liu, F. (2009). Detection and subtyping of influenza A virus based on a short oligonucleotide microarray. Diagnostic Microbiology and Infectious Diseases, 65, 261-70.
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