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何金生檢視原始碼討論檢視歷史

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何金生
北京交通大學

何金生,男,北京交通大學教授。

人物簡歷

教育背景

1986年7月畢業於安徽醫科大學臨床醫學專業,獲醫學學士學位,1994年7月畢業於安徽醫科大學基礎醫學院免疫學專業,獲免疫學碩士學位,2001年7月畢業於中國疾病預防控制中心,獲醫學博士學位,2004年10月至2005年10月加拿大多倫多大學訪問學者。

工作經歷

1986年7月起至2006年10月 歷任安徽醫科大學基礎醫學院微生物與免疫學學教研室助教、講師、副教授和教授,主要從事微生物學及免疫學教學及科研工作。

2006年11月至今 任北京交通大學理學院教授、理學院生命科學與生物工程研究院常務副院長,主要從事生物學科建設及生物學的教學和科研工作。

一直從事病毒新型疫苗和神經退行性疾病免疫治療研究,主持「十二五」傳染病重大專項1項,承擔「十二五」新藥創製重大專項1項。主持國家自然科學基金資助項目6項,主持北京市自然科學基金和教育部留學回國人員科研啟動基金資助項目各1項,承擔「十一五」863計劃「疫苗與抗體工程」重大項目「抗心腦血管凝血抗炎及抗病毒感染單抗藥物的研製及應用」子課題1項。同時主持與企業合作開展的研發項目2項。 以通訊或第一作者發表論文100篇,SCI論文40篇。獲得授權國家發明專利10項。轉讓1項發明專利。

研究領域

一、腺病毒載體疫苗及RNA病毒減毒活疫苗

(1)建立了RSV候選疫苗的減毒活性評價、系統及黏膜免疫效果、保護作用和機制研究平台;

(2)建立了人血清稀有型和非人靈長類腸道腺病毒基因組克隆及拯救平台,發現以腺病毒為載體的重組RSV疫苗,經滴鼻途徑免疫小鼠能誘導產生有效的免疫保護作用;

(3)建立了RSV病毒全基因組cDNA克隆及重組病毒拯救平台,以RSV Long株為親本,成功拯救獲得了可表達綠色熒光蛋白(EGFP)的重組RSV,以及分別攜帶cp、cpts、cptsdeltaSH和deltaM2-2等攜帶減毒和基因缺失聯合突變的減毒病毒,並完成了體內外系統研究;

(4)建立了以重組腺病毒-Vero細胞為表達系統的、RSV哺乳動物細胞病毒樣顆粒(VLP)製備平台,並完成了體內外系統研究;

二、阿爾茨海默病

(5)建立了可用於腦脊液和血液中A beta42診斷的雙抗體夾心ELISA診斷試劑盒,發現一個中國家族性AD存在的APPK724M突變,該突變影響APP在細胞內的代謝,能產生更高水平的A beta42,是導致早髮型FAD的致病因子。

研究方向

生物化學與分子生物學

物理與交叉學科

科研項目

  • 國家重大專項(民口)-負責:基於化學基因組學的病原診治生物標誌物高通量篩選平台,2013-01-01--2015-12-31,主持
  • 國家重大專項(民口)-負責:呼吸道合胞病毒減毒活疫苗研製,2013-01-01--2015-12-31,主持
  • 國家自然科學基金「面上」項目:嵌合SARS-CoV-2 S蛋白的人呼吸道合胞病毒減毒活疫苗研究,2021-2024,主持
  • 國家自然科學基金「面上」項目:人呼吸道合胞病毒複製缺陷型活疫苗與減毒活疫苗:免疫原性及安全性的比較研究,2018-2021,主持
  • 國家自然科學基金「面上」項目:提高以腺病毒載體為基礎的呼吸道合胞病毒疫苗安全性和有效性的方法研究,2010-01-01--2012-12-31,主持
  • 北京市自然基金「面上」項目:呼吸道合胞病毒疫苗初免-加強免疫策略的研究,2009-01-01--2011-12-31,主持
  • 科技部「863」:抗心腦血管凝血,抗炎及抗病毒感染單抗,2008-01-30--2010-12-31,主持
  • 國家自然科學基金「面上」項目:人呼吸道合胞病毒融合蛋白輔助病毒依賴型腺病毒重組體免疫保護作用,2007-01-01-2009-12-31,主持
  • 國家自然科學基金「面上」項目:可預防嬰幼兒呼吸道合胞病毒感染的口服DNA疫苗,2005-01-01-2007-12-31,主持
  • 國家自然科學基金「小額資助」:共表達RSV中和抗原的DNA和非複製型腺病毒重組體的構建及其保護作用,2004-01-01-2004-12-31,主持
  • 校科技基金:可表達RSV F蛋白的DNA疫苗和非複製型重組腺病毒免疫保護作用研究,2007-01-01--2008-12-31,10.0萬元,主持
  • 國家重大專項(民口)-參加:我國傳染病監測預警新策略與新技術研究,2010-02-20--2011-12-31,參加
  • 科技部「863」:乳腺細胞模型的建立及候選基因功能分析,2009-12-01--2010-10-31,參加
  • 科技部「863」:抗心腦血管凝血,抗炎及抗病毒感染單抗-抗Prion抗體藥物,2008-01-30--2010-12-31,參加
  • 北京交通大學:腫瘤基因治療的研究,2006-12-01--2011-12-31,參加

學術成果

論文/期刊

Jie‑Mei Yu, Yuan‑Hui Fu, Xiang‑Lei Peng, Yan‑Peng Zheng & Jin‑Sheng He. Genetic diversity and molecular evolution of human respiratory syncytial virus A and B, Scientific Reports (2021) 11:12941, https://doi.org/10.1038/s41598-021-92435-1

Lingwan Xu, Shijun Tian, Xianglei Peng, Ying Hua, Wenxuan Yang, Longwei Chen, Shilei Liu, Wenzheng Wu, Jiang Zhao, Jinsheng He, Liqing Wu, Jingfa Yang, Yanpeng Zheng. Clusterin inhibits Aβ42 aggregation through a "strawberry model" as detected by FRET-FCS Journal of Neurochemistry, 2021, DOI: 10.1111/jnc.15344 Li-Nan Wang, Xiang-Lei Peng, Min Xu, Yuan-Bo Zheng, Yue-Ying Jiao, Jie-Mei Yu, Yuan-Hui Fu, Yan-Peng Zheng, Wu-Yang Zhu, Zhong-Jun Dong, Jin-Sheng He, Evaluation of the Safety and Immune Efficacy of Recombinant Human Respiratory Syncytial Virus Strain Long Live Attenuated Vaccine Candidates. Virologica Sinica, 2021, DOI: 10.1007/s12250-021-00345-3

Minghua Wang, Guoning Zhang, Jianyuan Zhao, Ningning Cheng, Yujia Wang, Yuanhui Fu, Yanpeng Zheng, Juxian Wang, Mei Zhu, Shan Cen, Jinsheng He, Yucheng Wang. Synthesis and antiviral activity of a series of novel quinolone derivatives as anti-RSV or anti-IAV agents. European Journal of Medicinal Chemistry, 214 (2021) 113208, DOI: 10.1016/j.ejmech.2021.113208 Jie-Mei Yu, Li-Shu Zhang, Yuan-Hui Fu, Feng-Min Ji, Han-Li Xu, Jia-Qiang Huang, Xiang-Lei Peng, Yan-Peng Zheng, Ying Zhang and Jin-Sheng He. Analysis of Continuous Mutation and Evolution on Circulating SARS-CoV-2. Evolutionary Bioinformatics, 2020, Volume 16: 1–6, DOI: 10.1177/1176934320954870

Guo-Ning Zhang, Qiang Li, Jianyuan Zhao, Xuandi Zhang, Zhuxin Xu, Yujia Wang, Yuanhui Fu, Qi Shan, Yanpeng Zheng, Juxian Wang, Mei Zhu, Ziqiang Li, Shan Cen, Jinsheng He, Yucheng Wang. Design and synthesis of 2-((1H-indol-3-yl)thio)-N-phenyl-acetamides as novel dual inhibitors of respiratory syncytial virus and influenza virus A. European Journal of Medicinal Chemistry, 186 (2020) 111861 Shuo Xiao, Lin-Lin Song, Jiang-Tao Li, He Wang, Na Yu, Zi-Qi Wang, Ying Zhang, Jin-Sheng He, and Tao Hung. Intraperitoneal Administration of Monoclonal Antibody Against Pathologic A42 Aggregates Alleviated Cognitive Deficits and Synaptic Lesions in APP/PS1 Mice. Journal of Alzheimer’s Disease, 73 (2020) 657–670, DOI 10.3233/JAD-190874

Yuan-Hui FU; Zhu-Xin XU; Nan Jiang; Yan-Peng Zheng; Marie-Anne Rameix-Welti; Yue-Ying Jiao; Xiang-Lei Peng; Ye Wang; Jean-Francois Eleouet; Shan Cen; Jinsheng He. High-throughput screening of active compounds against human respiratory syncytial virus. Virology, 535 (2019) 171–178

Zheng, Yanpeng; Xu, Lingwan; Yang, Jingfa; Peng, Xianglei; Wang, He; Yu, Na; Hua, Ying; Zhao, Jiang; He, Jinsheng; Hong, Tao. The effects of fluorescent labels on Abeta42 aggregation detected by fluorescence correlation spectroscopy. Biopolymers, e23237, 2018-Oct-11, DOI: 10.1002/bip.23237 Zhang, Xiujuan; Ding, Xiaohui; Zhu, Yuanmei; Chong, Huihui; Cui, Sheng; He, Jinsheng; Wang, Xinquan; He, Yuxian. Structural and functional characterization of HIV-1 cell fusion inhibitor T20. AIDS (London, England) , 2018-Aug-08, DOI: 10.1097/QAD.0000000000001979 Xiujuan Zhang, Yuanmei Zhu, Hao Wu, Senyan Zhang, Pengfei Wang, Huihui Chong, Jinsheng He, Xinquan Wang, Yuxian He. Structural Insights into the Mechanisms of Action of Short-Peptide HIV-1 Fusion Inhibitors Targeting the Gp41 Pocket. Frontiers in Cellular and Infection Microbiology 2018, 8:51. doi: 10.3389/fcimb.2018.00051 Yao Ma, Yue-Ying Jiao, Yun-Zhou Yu, Nan Jiang, Ying Hua, Xiu-Juan Zhang, Yuan-Hui Fu, Xiang-Lei Peng, Yan-Peng Zheng, Larry J. Anderson, and Jin-Sheng He. A Built-In CpG Adjuvant in RSV F Protein DNA Vaccine Drives a Th1 Polarized and Enhanced Protective Immune Response. Viruses 2018, 10, 38; doi:10.3390/v10010038.

Min Xu, Yue-Ying Jiao, Yuan-Hui Fu, Nan Jiang, Yuan-Bo Zheng, Yi-Fei Yan, Mei Zhang, Yan-Peng Zheng, Wu-Yang Zhu, Xiang-Lei Peng, Jin-Sheng He. Construction and characterization of a recombinant human respiratory syncytial virus encoding enhanced green fluorescence protein for antiviral drug screening assay. BioMed Research International, Volume 2018, Article ID 8431243, 8 pages,https://doi.org/10.1155/2018/8431243

Yue-Ying Jiao, Yuan-Hui Fu, Yi-Fei Yan, Ying Hua, Yao Ma, Xiu-Juan Zhang, Jing-Dong Song, Xiang-Lei Peng, Jiaqiang Huang, Tao Hong, Jin-Sheng He. A single intranasal administration of virus-like particle vaccine induces an efficient protection for mice against human respiratory syncytial virus. Antiviral Research, 2017,144: 57-69. Ying Hua, Yue-Ying Jiao, Yao Ma, Xiang-Lei Peng, Yuan-Hui Fu, Xiu-Juan Zhang, Yuan-Bo Zheng, Yan-Peng Zheng, Tao Hong, Jin-Sheng He. Enhanced humoral and CD8 + T cell immunity in mice vaccinated by DNA vaccine against human respiratory syncytial virus through targeting the encoded F protein to dendritic cells. International Immunopharmacology, 2017, dx.doi.org/10.1016/j.intimp.2017,46:62-69 Yuan-Hui Fu Ya-Ru Liu Yan-Peng Zheng Nan Jiang Yue-Ying-Jiao Yao Ma Wei Li Xiang-lei Peng Jinsheng He. An RNA polymerase I-driven human respiratory syncytial virus minigenome as a tool for quantifying virus titers and screening antiviral drug. Chinese Chemical Letters, 2017, 28: 131-135

Ying Hua Yue-Ying Jiao Yao Ma Xiang-Lei Peng Yuan-Hui Fu Yan-Peng Zheng Tao Hong Jin-Sheng He. DNA Vaccine Encoding Central Conserved Region of G Protein Induces Th1 Predominant Immune Response and Protection from RSV Infection in Mice. Immunology Letters, 2016,179:95-101

Zheng, YP; Tian, SJ; Peng, XL; Yang, JF; Fu, YH; Jiao, YY; Zhao, J; He, JS; Hong. Kinesin-1 inhibits the aggregation of amyloid-beta peptide as detected by fluorescence cross-correlation spectroscopy. FEBS LETTERS, 2016, 590(7): 1028-1037 Lihua Guo, Hua Bai, Dongling Zou, Tao Hong, Jie Liu, Jiaqiang Huang, Pengfei He, Qi Zhou, and Jinsheng He. The role of microRNA-133b and its target gene FSCN1 in gastric cancer. Journal of Experimental & Clinical Cancer Research, 2014, 33:99, doi:10.1186/s13046-014-0099-0 Xianglei Peng, Lei Hou, Shaohua Xu, Ying Hua, Shujie Zhou, Ying Zhang, Yanpeng Zheng, Yuanhui Fu, Qing Xu, Lishu Zhang, Jun Wang, Xiaoting Guan, Jinsheng He. Novel APP K724M mutation causes Chinese EOFAD and increases amyloid-β42 to 40 ratio. Neurobiology of aging, 2014, 35(11):2657e1-2657e6, DOI:10.1016/j.neurobiolaging.2014.06.005

Yuan-Hui Fu Yue-Ying Jiao Jinsheng He Gui-Yun Jiang Wei Zhang Yao Ma Ying Zhang Chang-Xin Shi Xiang-Lei Peng Tao Hong Yi-Fei Yan. Sublingual administration of a helper-dependent adenoviral vector expressing the codon-optimized soluble fusion glycoprotein of human respiratory syncytial virus elicits protective immunity in mice. Antiviral Research 105 (2014), 72-79, DOI: 10.1016/j.antiviral.2014.02.003

Li-Hua Guo, Hui Li, Fang Wang, Jia Yu, and Jin-Sheng He. The Tumor Suppressor Roles of miR-433 and miR-127 in Gastric Cancer. Int. J. Mol. Sci. 2013, 14, 14171-14184;doi:10.3390/ijms140714171 Yuan-hui Fu, Jin-sheng He, Wei Qiao, Yue-ying Jiao, Ying Hua, Ying Zhang, Xiang-Lei Peng, and Tao Hong. Intranasal immunization with a helper-dependent adenoviral vector expressing the codon-optimized fusion glycoprotein of human respiratory syncytial virus elicits protective immunity in BALB/c mice. Virol J, 2013, 10:183. DOI: 10.1186/1743-422X-10-183 Zheng YP, Tang Y, Fu YH, He JS,Wang XB, Hong T. Purification of human respiratory syncytial virus fusion glycoprotein. Protein Expr. Purif, 2012, 81(1): 115-118

Lv RJ, He JS, Fu YH, Zhang YQ, Shao XQ, Wu LW, Lu Q, Jin LR, Liu H. ASIC1a polymorphism is associated with temporal lobe epilepsy. Epilepsy Res, 2011, 96(1-2): 74-80

Lv RJ, He JS, Fu YH, Shao XQ, Wu LW, Lu Q, Jin LR, Liu H. A polymorphism in CALHM1 is associated with temporal lobe epilepsy. Epilepsy Behav, 2011, 20(6): 681-685

Zhang Y, He JS, Guo SH, et al.Morphology and structural dynamics of amyloid beta 42 assembly in vitro. Neural Regeneration Research, 2011, 6(5): 335-339

Ying Zhang, Jin-Sheng HE, Xin WANG, Jun Wang, Fu-Xiang BAO, Si-Yuan Pang, Fan Yin, Tao Hong. Administration of Amyloid-beta42 Oligomer-Specific Monoclonal Antibody Improved Memory Performance in SAMP8 Mice. J Alzheimers Dis, 2011, 23 (3): 551-561

Yuan-hui Fu, Rui-juan Lv, Li-ri Jin, Qiang Lu, Xiao-qiu Shao, Jin-sheng He, Li-wen Wu, Li-shu Zhang, Hong-gang Hu. Association of apolipoprotein E polymorphisms with temporal lobeepilepsy in a Chinese Han population. Epilepsy Res, 2010, 91(2-3): 253-259 HE JinSheng, ZHANG Ying, HONG Tao. Progress in the development of therapeutic .antibodies targeting prion proteins and β-amyloid peptides.Sci China Life Sci, 2010, 53(8): 959-963 ZhENG YanPeng, HE JinSheng & HONG Tao. Biomarkers of Alzheimer’s disease in body fluids. Sci China Life Sci, 2010, 53(4): 490-496

Yuan-hui Fu, Jin-sheng He, Xiao-bo Wang, Xian-Xian Zheng, Qiang Wu, Can Xie, Wei Wei, Qian Tang, Jing-Dong Song, Jian-Guo Qu, Tao Hong. A prime–boost vaccination strategy using attenuated Salmonella typhimurium and a replication-deficient recombinant adenovirus vector elicits protectiveimmunity against human respiratory syncytial virus. Biochem Biophys Res Commun, 2010, 395(1): 87-92

Yuanhui Fu, Jinsheng He, Xianxian Zheng, Xiao-bo Wang, Can Xie, Chang-xin Shi, Mei Zhang, Qian Tang, Wei Wei, Jian-guo Qu and Tao Hong. Intranasal vaccination with a helper-dependent adenovirus vector enhances transgene-specific immune responses in BALB/c mice. Biochem Biophys Res Commun, 2010, 391(1): 857-861 Zhang Ying, Wang Xin, He Jin-sheng, Bao Fu-Xiang, Sun Wei-Min, Dai Xin-Xian, Wang Xiao-Bo, Li Yi-Qin, Zhen Xian-Xian, Hu Hong-Gang, Peng Xiang-Lei, Zheng Yan-Peng, Hou Ling-Ling, and Hong Tao. Preparation and Characterization of a Monoclonal Antibody with High Affinityfor Soluble Ab Oligomers. Hybridoma, 2009, 28 (5): 349-354

Yuanhui Fu, Jinsheng He, Xianxian Zheng, Qiang Wu, Mei Zhang, Xiaobo Wang, Yan Wang, Can Xie, Qian Tang, Wei Wei, Min Wang, Jingdong Song, Jianguo Qu, Ying Zhang, Xin Wang, Tao Hong. Intranasal Immunization with a Replication-Deficient Adenoviral Vector Expressing the Fusion Glycoprotein of Respiratory Syncytial Virus Elicits Protective Immunity in BALB/c Mice. Biochem Biophys Res Commun, 2009, 381(4):528-532 Can Xie, Jin-sheng He, Mei Zhang, Shao-Li Xue, Qiang Wu, Xiang-Dong Ding, Wei Song, Yuan Yuan, Dong-Liang Li, Xian-Xian Zheng, Yan-Yan Lu and Zhi Shang. Oral RSV DNA Vaccine expressing RSV F Protein Delivered by Attenuated Salmonella Typhimurium. Human Gene Therapy, 2007, 18(8):746-752

論著

1. 中華醫學百科全書生物藥物學卷,陳志南(主編),何金生等(編委),中國協和醫科大學出版社,2017,ISBN 978-7-5679-0670-9

2. 國家科學技術學術著作出版基金資助出版,《傳染性與非傳染性痴呆症:朊病毒病與阿爾茨海默病》,第1版,副主編,科學出版社,2011,ISBN 978-7-03-030345-5/R749.1

教材

1.《自然科學與技術研究方法》,第1版,編委,北京交通大學出版社,2012,ISBN 978-7-5121-0897-4/N.8

2.普通高等教育「十一五」國家規劃教材,全國高等醫學院校教材——《醫學免疫學》,第2版,編委,北京大學醫學出版社,2009,ISBN,978-7-81116-798-6

3.《免疫學雙語教學實驗技術指導》,副主編,科學出版社出版,2004,ISBN 7-03-012303-4/R.1191

專利

發明人:何金生 馬堯 付遠輝;中國發明專利:一種呼吸道合胞病毒融合前F蛋白及其應用,申請日:2019年4月25日,專利號:ZL 201910337141.5,授權公告日:2021年9月10日

發明人:何金生 彭向雷 付遠輝 許敏;中國發明專利:基於反向遺傳學構建的RSV反基因組質粒及其應用,申請日:2017年7月14日,專利號:ZL 201710574632.2,授權公告日:2020年3月5日

發明人:何金生 王玉成 付遠輝 張國寧 徐竹欣 李強 鄭妍鵬 王菊仙 彭向雷 白曉光;中國發明專利:一類3-位官能團化的N(O,S)-雜茚類衍生物及其在抗呼吸道合胞病毒中的應用,申請日:2018年02月05日,專利號:ZL 201810109813.2,授權公告日:2020年9月24日

發明人:何金生 付遠輝 彭向雷 鄭研鵬 姜男 許敏;中國發明專利:一種呼吸道合胞病毒藥物的高通量篩選方法和應用,申請日:2017年04月07日,專利號:ZL201710244050.8,授權公告日:2019年7月4日

發明人:何金生 付遠輝 焦月盈 洪濤:中國發明專利:一種呼吸道合胞病毒病毒樣顆粒及其製備方法和應用,專利號:ZL 201310667523.7,授權公告日:2016年8月24日,IPC分類號: C12N7/04; C12N15/45; C12N15/861; C07K16/10; A61K39/155; A61P31/14; G01N33/569

發明人:張瑩,何金生,宋琳琳,洪濤;中國發明專利:一種6´His-C99重組蛋白及其製備方法和應用,專利號:ZL 2012 1 0187669.7,授權公告日:2014年5月7日,IPC分類號:C07K19/00

發明人:張瑩,何金生,張娜,洪濤;中國發明專利:一種羧基末端特異的抗人澱粉樣蛋白單克隆抗體基因和其編碼多肽及應用,專利號:ZL 2012 1 0133385.X,授權公告日:2014年5月7日,IPC分類號:C12N15/13

發明人:何金生,張瑩,王鑫,洪濤;中國發明專利:抗阿爾茨海默病單克隆抗體及其應用,申請號:200910082077.7,申請日:2009年4月22日,專利號:ZL 2009 1 0082077.7,授權公告日:2012年7月18日,專利權人:北京交通大學,IPC分類號:C07K16/18

發明人:何金生;王鑫;張瑩;洪濤。中國發明專利:抗人澱粉樣蛋白單克隆抗體重輕鏈可變區基因及其應用,申請號:200910082076.2,申請日2009年4月22日,專利號:ZL 2009 1 0082076.2,授權公告日:2011年4月20日,專利權人:北京交通大學,IPC分類號:C12N15/13

發明人:王健偉;何金生;洪濤。中國發明專利:用於預防嬰幼兒腹瀉的重組腺病毒,申請號:01131084.7,申請日 2001年9月20日,專利號:ZL 01131084.7,授權公告日:2004年5月26日,IPC分類號:C12N7/01

發明人:王健偉;何金生;洪濤;徐倏燊。中國發明專利:表達人輪狀病毒VP7的重組腺病毒及製備方法和用途,申請號:02104125.3,申請日 2002年3月8日,專利號:ZL 02104125.3,授權公告日:2004年8月11日,IPC分類號:C12N7/01[1]

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